Catalog # CK7720

Cytoskeletal Filament Staining Kit

Immunocytochemistry Kit

Application / Dilution
ELISA, WB, IP, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Ck, F

$355


DATASHEET  

KIT SUMMARY

The cytoskeletal filament kit can be used to label the major cytoskeletal elements in cells: actin filaments (Phalloidin:FITC), intermediate filaments (Vimentin), and microtubules (α-tubulin). The kit includes Phalloidin:FITC and Goat anti-mouse conjugated to DyLight® 594 for for dual labeling experiments.

Immunocytochemical labeling of vimentin (VM4341; left), Phalloidin:FITC (PF7501; middle), and α-Tubulin (TM4111; right). The antibodies were detected using Goat anti-Mouse conjugated to DyLight® 594.



Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

TM4111 Gehrig, S.M. et al. (2012) Nature. 484(7394):394. WB: mouse skeletal muscle

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
Phalloidin:FITC Reagent100 μlICC, IHCHu, Rt, Ms, Ck, F1:500
α-Tubulin (C-terminus)Mouse mAb 50 μlWB, E, IP, ICC, IHCHu, Rt, Ms1:200
Vimentin Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms, Ck1:100
Anti-Mouse Ig:DyLight® 594 Goat pAb100 μlICC, IHCMs1:200