Catalog # CK7720

Cytoskeletal Filament Staining Kit

Immunocytochemistry Kit



The cytoskeleton includes actin filaments, microtubules, and intermediate filaments. These major cytoskeletal filaments are important for cell motility, cell adhesion, and cell morphology. Actin exists in two principal forms, globular, monomeric G-actin and filamentous, polymeric F-actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. Intermediate filament (IF) proteins are a diverse family with distinct expression patterns and functions. One major IF protein is vimentin, which forms filaments in mesenchymal, endothelial, and hematopoietic cells.

Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Hermann, H. et al. (2009) J Clin. Invest. 119(7):1772.
Abeyweera, T.P. et al. (2009) J Biol Chem. 284(26):17648.

Immunocytochemical labeling of vimentin (VM4341; left), Phalloidin:FITC (PF7501; middle), and α-Tubulin (TM4111; right). The antibodies were detected using Goat anti-Mouse conjugated to DyLight® 594.

Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

TM4111 Gehrig, S.M. et al. (2012) Nature. 484(7394):394. WB: mouse skeletal muscle

This kit contains:

Phalloidin:FITC Reagent100 μlICC, IHCHu, Rt, Ms, Ck, F1:500
α-Tubulin (C-terminus)Mouse mAb 50 μlWB, E, IP, ICC, IHCHu, Rt, Ms1:200
Vimentin Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms, Ck1:100
Anti-Mouse Ig:DyLight® 594 Goat pAb100 μlICC, IHCMs1:200

The cytoskeletal filament kit can be used to label the major cytoskeletal elements in cells: actin filaments (Phalloidin:FITC), intermediate filaments (Vimentin), and microtubules (α-tubulin). The kit includes Phalloidin:FITC and Goat anti-mouse conjugated to DyLight® 594 for for dual labeling experiments.