Catalog # CK7700

Cell Structure Staining Kit

Immunocytochemistry Kit

DATASHEET  




$455

Some of the major cell structures that form eukaryotic cells include a plasma membrane, cytoskeleton, nuclear envelope, cell junctional complexes and extracellular adhesion sites. The cytoskeleton includes actin filaments, microtubules, and intermediate filaments. These major cytoskeletal filaments are important for cell motility, cell adhesion, and cell morphology. Focal adhesions are protein complexes localized to the inner side of the plasma membrane at sites of adhesion. These complexes are important for adhesion to other cells and to the extracellular matrix. Paxillin is an important component of focal adhesions, while β-catenin is an important part of cell-cell junctions. The nucleus is formed from intermediate filament proteins that form a nuclear envelope. Nucleoporin p62 is an important protein in the nuclear pores that facilitate protein transport across the nuclear envelope.

References
Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Hermann, H. et al. (2009) J Clin. Invest. 119(7):1772.
Abeyweera, T.P. et al. (2009) J Biol Chem. 284(26):17648

Immunocytochemical labeling of nucleoporin p62 (NM4361; top row left) paxillin (PM1071; top middle), β-Catenin (CM11811; top right) vimentin (VM4341; bottom left), Phalloidin:FITC (PF7501; bottom middle), α-Tubulin (TM4111; bottom right). The antibodies were detected using either Goat anti-Mouse conjugated to DyLight® 594 or DyLight® 488.



Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PM1071 Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. IF: mouse NIH3T3 fibroblasts
PM1071 Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 IF: B16-F1 cells
CM1181 Condello, S. et al. (2013) FASEB J 27(8):3100. WB & IHC: Ovarian Cancer Cells
CM1181 Yakubov, B. et al. (2013) Neoplasia 15(6):609. WB: Human Ovarian Cancer Cells
PM1071 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PM1071 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
TM4111 Gehrig, S.M. et al. (2012) Nature. 484(7394):394. WB: mouse skeletal muscle

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYICC DILUTION
β-Catenin Mouse mAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms1:250
Nucleoporin p62 (N-terminal region) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms, Ck1:50
Paxillin Mouse mAb 50 μlWB, E, IP, ICCHu, Rt, Ms, Ck1:100
Phalloidin:FITC Reagent100 μlICC, IHCHu, Rt, Ms, Ck, F1:500
α-Tubulin (C-terminus)Mouse mAb 50 μlWB, E, IP, ICC, IHCHu, Rt, Ms1:200
Vimentin Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms, Ck1:100
KIT SUMMARY

The cell structure labeling kit can be used to identify important cell structures found in eukaryotic cells. The kit includes a panel of antibodies that detect the following cell structures:

Actin Filaments = Phalloidin:FITC 
Cell Junctions = β-catenin 
Focal Adhesions= Paxillin
Intermediate Filaments = Vimentin
Microtubules = α-Tubulin
Nuclear Envelope = Nucleoporin p62