Some of the major cell structures that form eukaryotic cells include a plasma membrane, cytoskeleton, nuclear envelope, cell junctional complexes and extracellular adhesion sites. The cytoskeleton includes actin filaments, microtubules, and intermediate filaments. These major cytoskeletal filaments are important for cell motility, cell adhesion, and cell morphology. Focal adhesions are protein complexes localized to the inner side of the plasma membrane at sites of adhesion. These complexes are important for adhesion to other cells and to the extracellular matrix. Paxillin is an important component of focal adhesions, while β-catenin is an important part of cell-cell junctions. The nucleus is formed from intermediate filament proteins that form a nuclear envelope. Nucleoporin p62 is an important protein in the nuclear pores that facilitate protein transport across the nuclear envelope.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Hermann, H. et al. (2009) J Clin. Invest. 119(7):1772.
Abeyweera, T.P. et al. (2009) J Biol Chem. 284(26):17648
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:PM1071 Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. IF: mouse NIH3T3 fibroblasts
PM1071 Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 IF: B16-F1 cells
CM1181 Condello, S. et al. (2013) FASEB J 27(8):3100. WB & IHC: Ovarian Cancer Cells
CM1181 Yakubov, B. et al. (2013) Neoplasia 15(6):609. WB: Human Ovarian Cancer Cells
PM1071 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PM1071 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
TM4111 Gehrig, S.M. et al. (2012) Nature. 484(7394):394. WB: mouse skeletal muscle
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||ICC DILUTION|
|β-Catenin Mouse mAb||50 μl||WB, E, IP, ICC, IHC||Hu, Rt, Ms||1:250|
|Nucleoporin p62 (N-terminal region) Mouse mAb||50 μl||WB, E, ICC||Hu, Rt, Ms, Ck||1:50|
|Paxillin Mouse mAb||50 μl||WB, E, IP, ICC||Hu, Rt, Ms, Ck||1:100|
|Phalloidin:FITC Reagent||100 μl||ICC, IHC||Hu, Rt, Ms, Ck, F||1:500|
|α-Tubulin (C-terminus)Mouse mAb||50 μl||WB, E, IP, ICC, IHC||Hu, Rt, Ms||1:200|
|Vimentin Mouse mAb||50 μl||WB, E, ICC||Hu, Rt, Ms, Ck||1:100|
The cell structure labeling kit can be used to identify important cell structures found in eukaryotic cells. The kit includes a panel of antibodies that detect the following cell structures:
Actin Filaments = Phalloidin:FITC
Cell Junctions = β-catenin
Focal Adhesions= Paxillin
Intermediate Filaments = Vimentin
Microtubules = α-Tubulin
Nuclear Envelope = Nucleoporin p62