Catalog # BL7011


Mouse Brain

Lysate

Application Dilution
WB20 μl/lane

Size 100 μl

$75

Brain (cerebrum and cerebellum) was dissected from adult mouse (Balb/c). The tissue was rinsed in Hank’s buffered saline solution and lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Brain lysates were sonicated then diluted to the final concentration in electrophoresis sample buffer.

 

References
MacPhee, D.J. (2010) J Pharmacol Toxicol Methods. 61(2):171.
Burgoyne, R.D.et al. (1988) EMBO J. 7(8):2311.
Dennis-Sykes, C.A. et al. (1985) J Biol Stand. 13(4):309.
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY