Catalog # AK6060


Actin & Tubulin

Antibody Sampler Kit

$395

Microtubules (MTs) and actin filaments are cytoskeletal elements that play an essential role in cell division, cytoplasmic organization, cell morphology, and motility. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. Actin filaments are polymers formed by one of six different actin isoforms found in vertebrates. Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. Phosphorylation may regulate the activity tubulin and actin isoforms. Cdk1 phosphorylation of Ser-172 in β-tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules. In Dictyostelium, actin phosphorylation at Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.

References
Baba, T. et al. (2003) J. Immunol. 170: 3762.
Winder, S.J. et al. (2005) J. Cell Sci. 118:651.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694.
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

AM2021 Pritchard, AJ et al. (2014) PLoS One. 9(6):e99444 WB: mouse splenocytes
TM1541 Wang, Q. et al. (2014) J Virol. 88(9):4853 WB: HeLa
AM2021 Dutta, P et al. (2014) J Neurochem. 130(3):360 WB: rat brain
AM2021 Muirhead, G et al. (2014) J Mol Neurosci. 53(1):125 WB: rat brain
AP1671 Vonach, C. et al. (2011) British J Cancer. 105:263. WB: human endothelial cells
TM1541 Meacham, CE. et al. (2011) Nature Genetics. 41:1133. WB: mouse lymphoma
TM1541 Meshki, J. et al. (2011) PLoS ONE. 6(9):e25332. WB: U3&3MG
TM1541 Campbell, S. et al. (2010) J Biol Chem. 285:4695. WB: human HeLa cells

Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-β-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-β-Tubulin (Ser-172) (lanes 3 & 4), and anti-β-Tubulin (TM1541) (lanes 5 & 6).

Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide (AX1655) or phospho-Actin (Tyr-53) peptide (AX1675), respectively (right).



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
Actin (N-terminal region) Rabbit pAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms, Ck42
Actin (Tyr-53), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck42
Actin (C-terminal region) Mouse mAb 50 μlWB, E, ICC, IHCHu, Rt, Ms, Ck42
β-Tubulin Mouse mAb50 μlWB, E, ICCHu, Rt, Ms50
β-Tubulin (a.a. 168-177) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck50
β-Tubulin (Ser-172), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck50
KIT SUMMARY

The actin and tubulin antibody sampler kit can be used to examine phosphorylation of actin (Tyr-53) and tubulin (Ser-172). In addition, the antibodies included in the kit can be used to detect actin and tubulin expression patterns by western blot or immunocytochemistry.