Microtubules (MTs) and actin filaments are cytoskeletal elements that play an essential role in cell division, cytoplasmic organization, cell morphology, and motility. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. Actin filaments are polymers formed by one of six different actin isoforms found in vertebrates. Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. Phosphorylation may regulate the activity tubulin and actin isoforms. Cdk1 phosphorylation of Ser-172 in β-tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules. In Dictyostelium, actin phosphorylation at Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.
Winder, S.J. et al. (2005) J. Cell Sci. 118:651.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:AM2021 Pritchard, AJ et al. (2014) PLoS One. 9(6):e99444 WB: mouse splenocytes
TM1541 Wang, Q. et al. (2014) J Virol. 88(9):4853 WB: HeLa
AM2021 Dutta, P et al. (2014) J Neurochem. 130(3):360 WB: rat brain
AM2021 Muirhead, G et al. (2014) J Mol Neurosci. 53(1):125 WB: rat brain
AP1671 Vonach, C. et al. (2011) British J Cancer. 105:263. WB: human endothelial cells
TM1541 Meacham, CE. et al. (2011) Nature Genetics. 41:1133. WB: mouse lymphoma
TM1541 Meshki, J. et al. (2011) PLoS ONE. 6(9):e25332. WB: U3&3MG
TM1541 Campbell, S. et al. (2010) J Biol Chem. 285:4695. WB: human HeLa cells
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|Actin (N-terminal region) Rabbit pAb||50 μl||WB, E, IP, ICC, IHC||Hu, Rt, Ms, Ck||42|
|Actin (Tyr-53), phospho-specific Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms, Ck||42|
|Actin (C-terminal region) Mouse mAb||50 μl||WB, E, ICC, IHC||Hu, Rt, Ms, Ck||42|
|β-Tubulin Mouse mAb||50 μl||WB, E, ICC||Hu, Rt, Ms||50|
|β-Tubulin (a.a. 168-177) Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms, Ck||50|
|β-Tubulin (Ser-172), phospho-specific Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms, Ck||50|
The actin and tubulin antibody sampler kit can be used to examine phosphorylation of actin (Tyr-53) and tubulin (Ser-172). In addition, the antibodies included in the kit can be used to detect actin and tubulin expression patterns by western blot or immunocytochemistry.