Catalog # SK6240

Sema-4D and Plexin B1 Receptor Antibody Kit

Antibody Sampler Kit

Application / Dilution

ELISA, WB, ICC, IHC, AB

Species Reactivity

Hu, Rt, Ms, Rb

KIT SUMMARY

The Sema-4D and Plexin B1 Receptor antibody sampler kit can be used to detect the changes in the expression of Semaphorin 4D and its receptor Plexin B1. The kit also includes a Plexin B1 peptide for antibody blocking experiments and secondary reagents for antibody detection.

Western blot image of mouse brain lysate. The blot was probed with mouse monoclonal Semaphorin-4D antibody (SM1881) at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

Immunocytochemical labeling in rat PC12 cells differentiated with NGF. The cells were probed with rabbit polyclonal Plexin B1 (PP1841) and mouse monoclonal Sema-4D (SM1881) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide, and the peptide is supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PP1841 Damola, A. et al. (2013) Prostate 73(12):1326. WB, IP: Prostate Cells, shRNA knockdown
PP1841 Soong, J. & Scott, G. (2013) J Cell Sci. 126(Pt 2):688. WB: human melanocytes
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
SM1881 Damola, A. et al. (2013) Prostate 73(12):1326. WB: prostate cancer cells
PP1841 Soong, J. et al (2012) J Invest Dermatol. 132(4):1230. WB: melanocyte, keratinocyte, smooth muscle
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG#	DESCRIPTION	SIZE	APPLICATIONS	SPECIES REACTIVITY	MW (kDa)
PP1841	Plexin B1 (C-terminal region) Rabbit pAb	50 μl	WB, E, ICC	Hu, Rt, Ms	300
PX1845	Plexin B1 (C-terminal region) Peptide	50 μg	AB, E
SM1881	Semaphorin-4D (C-terminal region) Mouse mAb	50 μl	WB, E, ICC	Hu, Rt, Ms	150
MS3001	Anti-Mouse Ig:HRP Donkey pAb	100 μl	WB, E	Ms
RS3251	Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb	100 μl	WB, E, ICC, IHC	Rb