Catalog # RS3201

Anti-Rabbit Ig:HRP Secondary Antibody

Goat Polyclonal

Application Dilution
ELISA1:10000
ICC1:1000
IHC1:1000
WB1:5000

Size 100 μl

Species Reactivity Rb

DATASHEET  




$55

An array of chromogenic, fluorogenic and chemiluminescent substrates are available for use with HRP conjugated secondary antibodies HRP is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or the release of light as a byproduct of the reaction. HRP functions optimally at a near-neutral pH and can be inhibited by cyanides, sulfides and azides. Antibody-HRP conjugates are superior to antibody-AP conjugates with respect to the specific activities of both the enzyme and antibody. In addition, its high turnover rate, good stability, low cost and wide availability of substrates makes HRP the enzyme of choice for most applications. HRP can be used for chemiluminescent readout in western blot, colorimetric readout in ELISA, and precipitation reactions useful in immunocytochemistry and immunohistochemistry.

 

References
Madamanchi, N.R. & Runge, M.S. (2001) Methods Mol Med. 51:245.
Mattson, D.L. & Bellehumeur, T.G. (1996). Anal. Biochem. 240:306.

Western blot analysis of A431cells treated with calyculin A (10 nM) for 30 min. The blot was probed with four different rabbit polyclonal antibodies (lane 1: anti-Akt (Thr-34), lane 2: anti-β-Catenin, lane 3: anti-N-WASP (Ser-484/Ser-485), lane 4: anti-Paxillin (Ser-178). This panel of primary antibodies was then detected using goat anti-rabbit Ig:HRP (1:5,000).



The HRP-conjugated goat polyclonal secondary reagent reacts with the heavy chains on rabbit IgG, and the light chains of most rabbit immunoglobulins. The secondary reagent has minimal cross-reactivity with human, mouse, and rat immunoglobulins.

Goat polyclonal antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, and 1 mg/ml BSA. Store at –20°C. Stable for 1 year. Note: Use of sodium azide as preservative substantially inhibits the enzyme activity of HRP.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody has been pre-adsorbed with various immunoglobulins from non-rabbit species before affinity-purification using rabbit IgG coupled to agarose beads. Purified goat polyclonal antibody was conjugated to horseradish peroxidase (HRP). This secondary reagent can be used to detect rabbit immunoglobulins in various antibody applications that utilize HRP readouts, such as western blot, ELISA, immunocytochemistry, and immunohistochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY