Catalog # SP1531

SHP1 (Ser-591), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 68 kDa

SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKCα and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.

References

Jones, M.L. et al. (2004) J. Biol. Chem. 279(39):40475.
Zhang, Z. et al. (2003) J. Biol. Chem. 278(7):4668.
Zhang, J. et al. (2000) Semin. Immunol. 12:361.

Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Ser-591) antibody (lanes 3-6). The SHP1 (Ser-591) antibody was used in the presence of phospho-SHP1 (Ser-591) peptide (lane 5) or a non-specific phospho-serine peptide (lane 6).

Phospho-SHP1 (Ser-591) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 591 in human SHP1. The sequence is highly conserved in rat and mouse SHP1.

*For more information, see UniProt Accession P29350

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to a non-specific phospho-serine peptide then affinity-purified using phospho-SHP1 (Ser-591) peptide. The antibody detects a 68 kDa* band on SDS-PAGE immunoblots of human Jurkat and A431 cells treated with pervanadate, as well as A431 cells treated with EGF.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Ormonde, JVS et al. (2018) J Immunol. 201(11):3431. (WB: human T cells)
Moogk D. et al. (2016) J Immunol. 197(2):644. (WB/FC: mouse T cells)
Mani, R. et al. (2015) Leukemia 29: 346. (WB: mouse CCL)
Tibaldi, E. et al. (2014) BBA 1843(2):288. (WB: hepatic stellate cells)
Kim, JH et al. (2013) Neurobiol Dis. 60:1. (WB: mouse astrocyte)
Zhou, X. et al. (2013) AJP Renal Phys. 305(3):362. (WB Li-Cor: HEK293 cells treated with NaCl )
Zonta, F. et al. (2014) Blood. 125(24):3747. (WB: human B-Cells)
Sanchez-Quiles, A. et al. (2012) J Proteomics. 75(18):5783. (WB: mouse splenocytes)
Salas, A. et al. (2011) Blood. 117(22):5941. (WB: human K562 cells)
Zhou, et al. (2010) Prco Natl Acad Sci. 107(15):7072. (WB: mIMCD3 cells)
Simoneau, M. et al. (2008) J Biol Chem. 283(37):25544. (WB: S591A transfectants)

This kit contains: