Catalog # CP1981

VE-Cadherin (Tyr-685), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 140 kDa

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.

References

Wallez Y. et al. (2007) Oncogene 26:1067.
Baumeister U. et al. (2005) EMBOJ 24:1686.
Potter M.D. et al. (2005) J Biol. Chem. 280(36):31906.

Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).

Phospho-VE-Cadherin (Tyr-685) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 685 in human VE-cadherin. This sequence has significant homology to the conserved site in rat and mouse VE-cadherin, but is not conserved in other cadherins.

*For more information, see UniProt Accession P33151

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to an unrelated phospho-tyrosine peptide and unphosphorylated VE-cadherin (Tyr-685) peptide before affinity purification using phospho-VE-cadherin (Tyr-685) peptide. The purified antibody detects a 140 kDa* band corresponding to VE-cadherin in western blots of human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Caolo, V et al. (2018) Arteriosc Throm Vasc Biol 38(9):2174. (WB: mouse embryos)
Wylezinski, LS et al. (2016) J Biol Chem. 291(44):22913. (WB: human, mouse BMECs)
Benn, A. et al. (2016) J Cell Sci. 129(1):206. (WB: HUVECs)
Sivaraj KK et al.(2015) Cardiovasc Res. 108(1):171-80 (WB: HUVEC)
Zhang, P. et al. (2014) FEBS Lett. 588(24):4573. (WB: human melanoma and HUVEC)
Cain, R. et al. (2012) Int J Biochem Cell Biol. 44(11):1929. (WB: HUVECs )
Heijden, M. et al. (2011) PLoS ONE 6(8):e23448. (WB: human pulmonary microvascular ECs )
Lo, CW et al. (2010) Cancer Res. 71(2):424. (WB: HUVECs)
Cain, R. J. et al. (2010) J Cell Biol. 188(6):863. (WB: HUVECs)
Adam, A.P. et al. (2010) J Biol Chem. 285(10):7045. (WB: HDMECs)
Fainaru, O. et al. (2008) FASEB J 10:3728. (WB: H. dermal microvascular ECs )

This kit contains: