Catalog # PP3891

PDK1 (Ser-241), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
IHC	1:50
WB	1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 65 kDa

3-Phosphoinositide-dependent kinase 1 (PDK1), also known as PKB kinase, was identified as the activator of the survival kinase Akt/PKB. Several important substrates of PDK1 include p70S6 kinase, PKAs, PKCs, SGKs, RSKs, and PAKs. PDK1 is a member of the AGC superfamily of serine/threonine kinases. Through the phosphorylation of downstream kinases like Akt, PDK1 has been shown to be involved in several different cell functions, such as protein synthesis, cell survival, glucose metabolism, and cell adhesion and migration. The regulation of PDK1 occurs through lipid second messengers and phosphorylation. Multiple serine sites are phosphorylated on PDK1. Serine 241 phosphorylation is required for PDK1 activity, while serine 396 has been implicated in PDK1 nuclear translocation. Tyrosine phosphorylation may also regulate PDK1 activity. Tyrosines 9 and 373/376 are phosphorylated by c-Src in vitro. Tyr-373/Tyr-376 is important for PDK1 activity, while Tyr-9 phosphorylation permits Tyr-373/Tyr-376 phosphorylation by c-Src. In addition, Tyr-9 may be important during angiotensin-II-induced focal adhesion formation.

References

Scheid, M.P. et al. (2005) Mol. Cell. Biol. 25(6):2347.
Taniyama, Y. et al. (2003) Mol Cell. Biol. 23(22):8019.
Park, J. et al. (2001) J Biol. Chem. 276(40):37459.

Western blot of MDA-MB-435 cells untreated (lanes 1 and 3) or treated with EGF (100 ng/ml) for 1 hr (lanes 2 & 4). Blots were probed with mouse monoclonal anti-PDK1 (C-terminal region) or rabbit polyclonal anti-PDK1 (Ser-241).

Phospho-PDK1 (Ser-241) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 241 in human PDK1. This sequence is highly conserved in rat and mouse PDK1.

*For more information, see UniProt Accession O15530

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using phospho-PDK1 (Ser-241) peptide. The purified antibody detects a 65 kDa* protein corresponding to the apparent molecular mass of PDK1 on SDS-PAGE immunoblots of human MDA-MB-435 cells treated with EGF, but not in untreated cells. PDK1 (Ser-241) reactivity was also observed by immunohistochemistry in human breast carcinoma tissue.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: