Catalog # PP1341

Paxillin (Ser-83), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:50
WB	1:1000

Size 100 μl

Species Reactivity Rt, Ms

MW 68 kDa

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both ERK and p38MAPK kinases phosphorylate serine 83 in vitro. HGF stimulation of murine epithelial cells leads to ERK-mediated phosphorylation of Ser-83, which is required for HGF-induced cell spreading and migration. In addition, Ser-83 is phosphorylated in response to NGF in PC12 cells, and this phosphorylation may be involved in neurite extension. In human paxillin, Ser-85 rather than Ser-83 may be the site phosphorylated by p38 MAPK and mutation of this site inhibits NGF-induced neurite extension. Thus, serine residues in the N-terminal region of paxillin may be important for growth-factor mediated changes in activity.

References

Huang, C. et al. (2004) J Cell Biol. 164(4):593-602.
Ishibe, S. et al. (2004) Mol. Cell 16 :257-267.

Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with NGF (200 ng/ml) for 60 min (lanes 2 & 4). The blots were probed with mouse monoclonal anti-Paxillin (PM1071; lane 1 & 2) or rabbit polyclonal anti-Paxillin (Ser-83) phospho-specific antibody (lane 3 & 4).

Immunocytochemical labeling of Ser-83 phosphorylated paxillin in rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal Paxillin (left) and rabbit polyclonal Paxillin (Ser-83, right) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3.

Phospho-Paxillin (Ser-83) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 83 of mouse paxillin. This sequence is highly conserved in rat and human paxillin and is also found in all isoforms (a, b, g) of paxillin.

*For more information, see UniProt Accession Q8VI36

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using phospho-Paxillin (Ser-83) peptide (without carrier). The antibody detects a 68kDa* protein corresponding to the molecular mass of phosphorylated paxillin on SDS-PAGE immunoblots of NGF treated rat PC12 cells, as well as calyculin A treated human A431 cells and mouse macrophages.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Fan, Y. et al. (2018) Front Cell Neurosci. 12:309. (WB: mouse BV2 microglia)
Fuste, NP et al. (2016) Nat Commun. 7:11581. (WB/ICC/IHC: rat tumor cells)
Wu, D. W. et al. (2014) Oncogene 33(35): 4385. (WB: CCM3 cells)
Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. (WB: rat C6 and human U373 gliomas)
Sen, A. et al. (2012) J Clin Invest. 122(7):2469. (WB, ICC: C4C2, PC3, LNCaP, )
Kwak, T.K. et al. (2010) J Biol Chem. 285(46):36021. (WB: pancreatic INS-1 cells)
Sen, A. et al. (2010) J Biol Chem. 285(37):28787. (WB: human LNCAP cells)

This kit contains: