Catalog # PP1051

Paxillin (Ser-178), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
WB	1:500
IHC	1:100

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 68 kDa

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.

References

Huang, C. et al. (2004) Cell Cycle 3(1):4-6.
Huang, C. et al. (2003) Nature 424:219-223.
Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.

Western blot analysis of A431 cells (20 µg/lane) serum starved overnight and treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-Paxillin mouse monoclonal (PM1071) or anti-Paxillin (Ser-178) rabbit polyclonal (PP1051).

Phospho-Paxillin (Ser-178) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.

*For more information, see UniProt Accession P49023

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 68kDa* protein corresponding to the molecular mass of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic endothelial cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Chen, M.J. et al. (2020) Am J Cancer Res. 10(1):275. (IHC, WB: S178A mutant)
Wu, D. W. et al. (2014) Oncogene 33(35): 4385. (WB: CCM3 cells)
Wei, W. et al. (2013) Pancreatology 13(4):384. (WB: pancreatic PANC1, Capan-2 and HT1080 cells)
Liu, H. et al. (2013) BMC Cancer. 13:80. (WB: human ovarian cancer cells)
Kwak, T.K. et al. (2010) J Biol Chem. 285(46):36021. (WB: pancreatic INS-1 cells)

This kit contains: