Catalog # PM2691

PYK2 (Tyr-402), phospho-specific Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:2000
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 116 kDa

Isotype IgG1

Protein tyrosine kinase, Pyk2 (CAKb, RAFTK, CADTK), is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration of various cell types. In T-cells, Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR. Phosphorylation of Tyr-402 is required for the phosphorylation of other tyrosines in Pyk2 and provides a binding site for Fyn SH2 during T-cell activation.

References

Duong, L. T. et al. (2001) J. Biol. Chem. 276:7484.
Katagiri, T. et al. (2000) J. Biol. Chem. 275:19645.
Avraham, S. et al. (1995) J. Biol. Chem. 270:27742.
Lev, S. et al. (1995) Nature 376:737.
Sasaki, H. et al. (1995) J. Biol. Chem. 270:21206.

Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-PYK2 (C-terminal region) antibody (lanes 1 & 2) or anti-PYK2 (Tyr-402) (lanes 3 & 4).

Clone M269 was generated from a phosphopeptide containing amino acids surrounding tyrosine 402 in human PYK2. This sequence is conserved in rat and mouse PYK2, and has significant homology to the conserved site in FAK.

*For more information, see UniProt Accession Q14289

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 116 kDa* band corresponding to PYK2 on SDS-PAGE immunoblots of human A431, Jurkat and K562 cells treated with pervanadate, but is not observed in control, untreated cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: