Catalog # MP3831

Myosin IIA Heavy Chain (Ser-1943), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 200 kDa

Non-muscle myosin II is an actin-based motor protein essential to cell motility, cell division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are three NMHC-II isoforms (NMHC-IIA, NMHC-IIB, and NMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation of NMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, leading to activation of myosin II motor activity and increased myosin filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. NMHC-II phosphorylation may be an important mode for regulating myosin-II assembly. PKC phosphorylates NMHC-IIA on Ser-1916 in the C-terminal region and NMHC-IIB on multiple serines in the tailpiece. Casein kinase II phosphorylates NMHC-IIA on Ser-1943 in the tailpiece and increases disassembly of NMHC-IIA filaments.

References

Breckenridge, M.T. et al. (2009) Mol Biol Cell 20:338.
Conti, M.A. & Adelstein, R.S. (2008) J Cell Sci. 121:11.
Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18:3144.
Murakami, N. et al. (1998) Biochemistry 37:1989.

Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).

Immunocytochemical labeling of myosin IIA heavy chain phosphorylation relative to F-actin in chick fibroblasts. The cells were labeled with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943) antibody (MP3831), then detected using appropriate secondary antibody (Bottom, Red). This labeling is compared to F-actin staining (Bottom, Green) and to secondary only (Top). (Image provided by Dr. Gianluca Gallo at Drexel University).

Phospho-NMHC-IIA (Ser-1943) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1943 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse NMHC-IIA, but is not found in NMHC-IIB and is not well conserved in NMHC-IIC.

*For more information, see UniProt Accession P35579

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to unphosphorylated NMHC-IIA (Ser-1943) peptide before affinity purification using phospho-NMHC-IIA (Ser-1943) peptide (without carrier). The antibody detects a 200 kDa* protein corresponding to NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells. This reactivity is removed after treatment with lambda phosphatase. The antibody also detects NMHC-IIA (Ser-1943) phosphorylation in chick Fibroblasts.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Cao, Y. et al. (2018) Exp Cell Res. 373(1-2):119. (WB: human HeLa)
Chen, Y. et al. (2016) Exp Cell Res. 349(1):68-76. (WB: human HepG2)
Guilbert, A. et al. (2013) Eur J Cancer. 49(17):3694. (WB: MDA-MB-231 cells)
Sanborn, K. et al. (2013) Blood 118(22):5862. (WB: human YTS cells)

This kit contains: