Catalog # NM0161

Na+/K+ ATPase β3 (Extracellular) M016 Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:2000
ICC	1:50
IP-MS	1:50
WB	1:1000

Size 100 μl

Species Reactivity Hu

MW 40 kDa

Isotype IgG1

The Na+/K+ ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving Na+ export and K+ import across the plasma membrane. It is composed of a large catalytic α subunit and a membrane-spanning auxiliary β subunit. In humans, the Na+/K+ ATPase is a binary complex of an α subunit that has four isoforms (α1-α4) and a β-subunit that has three isoforms (β1, β2, β3). Na+/K+ ATPase subunit expression has been shown to be upregulated in cancers, and inhibition of Na+/K+ ATPase activity has anti-cancer effects. The β3 subunit of Na+/K+ ATPase has increased expression in human gastric cancer tissues and cell lines, and its increased expression level predicts poor patient outcome. β3 subunit knockdown significantly inhibited cell proliferation, colony-formation ability, migration, and invasion in human gastric carcinoma cell lines.

References

Clausen, MV et al. (2017) Front Physiol. 8:371.
Li, L et al. (2017) Oncotarget. 8(48):84285.
Yoshimura, SH et al. (2008) J Cell Sci. 121:2159.

Western blot of NM0161 immunoprecipitates (IP) and whole lysates. The NM0161 antibody only (lane 1), IP from A431 cell lysate (lane 2), A431 cell input (lane 3), LNCaP cells (lane 4), MeWo cells (lane 5), and normal human lung (lane 6) and skin (lane 7). The blot was probed with anti-Na+/K+ ATPase β3 NM0161 (lanes 1-7). The arrow designates native antibody, while the β3 subunit migrates around 40 kDa.

Immunocytochemical labeling of Na+/K+ ATPase β3 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-Na+/K+ ATPase β3 (clone M016). The antibody was detected using goat anti-mouse Ig DyLight® 594.

Clone (M016) was generated from a proprietary antigen related to the native human Na+/K+ ATPase β3 subunit expressed in A431 epidermoid carcinoma cell line.

*For more information, see UniProt Accession P54709

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M016 mouse monoclonal antibody detects a 40 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase β3 subunit. In addition, mass spectrometry analysis of immunoprecipitates using NM0161 in human A431 cell lysates confirms that this antibody only detects Na+/K+ ATPase β3 protein.The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the β3 subunit on live cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: