Catalog # PK7690

p38 MAPK Phospho-Regulation Staining Kit

Immunocytochemistry Kit

Application / Dilution

ELISA, WB, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Ck, Rb

KIT SUMMARY

The p38 MAPK phospho-regulation kit can be used for immunocytochemical co-localization of p38 MAPK phosphorylation at Thr-180/Tyr-182 and Tyr-323 relative to the total expression of p38 MAPK. The kit includes rabbit polyclonal and mouse monoclonal antibodies along with Goat-anti-Rabbit conjugated to DyLight® 594 and Goat anti-Mouse conjugated to DyLight® 488 for dual labeling experiments.

Immunocytochemical labeling of p38 MAPK in control and pervanadate-treated mouse C2C12 cells. The cells were labeled with mouse monoclonal p38α MAPK (PM1381), p38 MAPK (Thr-180/Tyr-182) (PM1391), and rabbit polyclonal p38 MAPK (Tyr-323) (PP3411). The antibodies were detected using appropriate secondary conjugated to DyLight® 488 or DyLight® 594.

Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

PP3411 Alam, M.S. et al. (2015) Nat Medicine 21:1337. WB, IF: human T-cells, CD3 activated
PP3411 Lanna, A. et al. (2014) Nat Immunol. 15(10):965. WB: human T-cells, CD3 activated
PM1391 Chambers, M.A. et al. (2009) J Physiol. 587:3363. WB: mouse muscle, C2C12
PM1391 Li, W. et al. (2009) AJP Cell Phys. 297:C706. WB: mouse C2C12 cells

This kit contains:

CATALOG#	DESCRIPTION	SIZE	APPLICATIONS	SPECIES REACTIVITY	ICC DILUTION
PM1381	p38α MAP Kinase (C-terminal) Mouse mAb	50 μl	WB, E, ICC	Hu, Rt, Ms	1:200
PM1391	p38 MAP Kinase (Thr-180/Tyr-182), phospho-specific Mouse mAb	50 μl	WB, E, ICC	Hu, Rt, Ms	1:250
PP3411	p38α MAP Kinase (Tyr-323), phospho-specific Rabbit pAb	50 μl	WB, E, ICC	Hu, Rt, Ms, Ck	1:300
MS3011	Anti-Mouse Ig:DyLight® 488 Goat pAb	100 μl	ICC, IHC	Ms	1:200
RS3271	Anti-Rabbit Ig:DyLight® 594 Goat pAb	100 μl	ICC, IHC	Rb	1:200