Catalog # IK7251

Integrin β1 (Human) Paired ELISA KIT

ELISA Kit

Application / Dilution

ELISA

Species Reactivity

Human

KIT SUMMARY

The Integrin β1 Paired ELISA kit can be used for capture of human Integrin β1 protein in ELISA sandwich assays of cell lysates, serum, and other liquid biological samples. The kit includes mouse monoclonal anti-Integrin β1 (IM004P) capture antibody and anti-Integrin β1 (IM041D) Isotype IgG2a detection antibody along with anti-Mouse IgG2a specific:HRP (MS3221) secondary reagent.

Representative Standard Curve using mouse monoclonal anti-Integrin β1 (IM004P) for ELISA capture of human recombinant integrin α2/β1 heterodimer. Capture was detected using mouse monoclonal isotype IgG2a anti-Integrin β1 (IM041D) followed by anti-Mouse IgG2a specific:HRP.

Mouse monoclonal capture antibody is supplied in phosphate-buffered saline and 0.05% sodium azide. Mouse monoclonal detection antibody is supplied in phosphate-buffered saline, 50% glycerol, and 0.05% sodium azide. Anti-Mouse IgG2a:HRP is supplied in phosphate-buffered saline, 50% glycerol, and 1 mg/ml BSA. Store all at –20°C. Stable for 1 year

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

CATALOG#	DESCRIPTION	SIZE	APPLICATIONS	SPECIES REACTIVITY	DILUTION
IM004P	Integrin β1 (Extracellular region) Mouse mAb	50 μl	ELISA (Cap)	Hu	1:250
IM041D	Integrin β1 (Extracellular region) Mouse mAb	50 μl	ELISA (Det)	Hu	1:1000
MS3221	Anti-Mouse IgG2a specific:HRP Goat pAb	100 μl	Secondary	Ms	1:2000