![Cyclophilin B Antibody](http://ecmbio.com/cdn/shop/products/Icon_GOmAbred2_text2_fe14dbe7-937c-4de9-9932-73dc6a96af46_compact.png?v=1597166261)
Catalog # CM0191
Cyclophilin B Antibody
Mouse Monoclonal
ELISA | 1:2000 |
ICC | 1:100 |
IP | 1:100 |
WB | 1:1000 |
Size 100 μl
Species Reactivity Hu
MW 20 kDa
Isotype IgG1
Cyclophilins are a highly conserved family of peptidylprolyl cis-trans-isomerases (PPI) that are targets of the immunosuppressant drug cyclosporin A. These isomerases have been proposed to aid in protein folding. Cyclophilin B (PPIB) is an ER-localized chaperone protein that associates with type I procollagen, and facilitates procollagen sorting and transport through the secretory compartment. Mutations in the cyclophilin B gene lead to aberrant biosynthesis of type I procollagen, and osteogenesis imperfecta, a disorder characterized by bone fragility. Cyclophilin B has also been implicated as a marker in several cancers, including glioblastomas, neuroblastomas, and gastric cancers. In gastric cancer, Stat3 suppression of miR-520d-5p leads to upregulation of its downstream target cyclophilin B, and enables the growth and survival of these cancer cells.
References
Li, T et al. (2017) Cancer Res. 77(5):1227.
Pyott, SM et al. (2011) Hum Mol Genet. 20:1595 .
![](https://cdn.shopify.com/s/files/1/0016/1021/9589/files/cyclophilinB_CM0191.jpg)
Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (CM0191) at 1:500.
![](https://cdn.shopify.com/s/files/1/0016/1021/9589/files/CyclophilinB_CM0191_2c68ee6f-9adf-406d-b7cc-d2d54d4f142b.jpg?11339748804131525062)
Immunocytochemical labeling of cyclophilin B in methanol:acetone (1:1) fixed human A549 lung cancer cells. The cells were labeled with mouse monoclonal anti-cyclophilin B (CM0191). The antibody was detected using goat anti-mouse DyLight® 594.
*For more information, see UniProt Accession P23284
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains: