Catalog # EK6160

EGFR Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution

ELISA, WB, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Rb

KIT SUMMARY

The EGFR phospho-regulation antibody sampler kit can be used to detect EGFR phosphorylation at Ser-1142, Ser-967, and Tyr-1101. The kit also includes an antibody to examine total EGFR expression levels, and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.

Western blot image of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then probed with anti-EGFR (a.a. 961-972) (lanes 1 & 2), anti-EGFR (Ser-967) (lanes 3 & 4), or anti-EGFR (Ser-1142) (lanes 5 & 6).

Immunocytochemical labeling in A431 cells untreated or treated with EGF (100 ng/ml) for 5 min. The cells were labeled with anti-EGFR or anti-EGFR (Tyr-1101) monoclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy3.

Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

EM1991 Solis, N.V. et al. (2017) MBio. 8(2). pii: e00025-17. WB:human epithelial cells
EP1931 Jhaveri, T.J. et al. (2015) Oncotarget. 6(17):14754 WB: HCC1806 cells
EP1931 Kamekura, R. et al. (2014) Oncogene. 33(36): 4531. WB: human SK-CO15
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
EP1871 Choi, S. et al. (2012) Am J Pathol. 180(1):410. WB: mouse skeletal muscle
EP1871 Kolegraff, K. et al. (2011) Mol Biol Cell. 22(8):1121. WB: human intestinal epithelial cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

CATALOG#	DESCRIPTION	SIZE	APPLICATIONS	SPECIES REACTIVITY	MW (kDa)
EP1871	EGFR (a.a. 961-972) Rabbit pAb	50 μl	WB, E, ICC	Hu, Rt, Ms	180
EP1931	EGFR (Ser-1142), phospho-specific Rabbit pAb	50 μl	WB, E, ICC	Hu, Rt, Ms	180
EP1911	EGFR (Ser-967) Rabbit pAb	50 μl	WB, E	Hu, Rt, Ms	180
EM1991	EGFR (Tyr-1101), phospho-specific Mouse mAb	50 μl	WB, E, ICC	Hu, Rt, Ms	180
MS3001	Anti-Mouse Ig:HRP Donkey pAb	100 μl	WB, E	Ms
RS3251	Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb	100 μl	WB, E, ICC, IHC	Rb