Catalog # TM3391

TRADD (C-terminal region) Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:2000
ICC	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 34 kDa

Isotype IgG1

Tumor necrosis factor (TNF) activates TNF receptor-1 (TNFR1), leading to diverse biological activities in many cell types. TNFR1 contains an intracellular death domain that is required for signaling related to antiviral activity, programmed cell death, and NF-κB activation. TNFR1-associated death domain protein (TRADD) was isolated in a screen to identify proteins that interact with the death domain of TNFR1. TRADD is a 34-kDa protein that contains a 111-amino acid death domain with sequence similarity to TNFR1. This domain mediates oligomerization of TRADD and interaction with TNFR1. TRADD mRNA is expressed ubiquitously, and overexpression of TRADD causes TNF-induced apoptosis and activation of NF-κB. These responses require the TRADD death domain. In addition to TNF signaling, TRADD N-terminus can interact with Stat1 in response to IFNγ, and this interaction can negatively regulate Stat activity.

References

Wesemann, D.R. et al. (2004) Nat. Immun. 5:199.
Hsu, H. et al. (1995) Cell 81:495.

Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal TRADD (C-terminal region) antibody at 1:250 (lane 1) or 1:1000 (lane 2).

Immunocytochemical labeling of TRADD in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with mouse monoclonal TRADD (C-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.

Clone M339 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human TRADD. This sequence has high homology to similar regions in rat and mouse TRADD.

*For more information, see UniProt Accession Q15628

Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 34 kDa* protein corresponding to the apparent molecular mass of TRADD on SDS-PAGE immunoblots of adult mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: