Catalog # TK6600

α/β-Tubulin Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution

Species Reactivity

Hu, Rt, Ms, Ck, F




The α/β-tubulin phospho-regulation kit can be used to examine phosphorylation of α-tubulin (Ser-165) and β-tubulin (Ser-172) relative to the total expression of these tubulin proteins. In addition, the kit has an antibody to detect unphosphorylated βIII-tubulin (Ser-444). The kit includes mouse monoclonal antibodies to detect the total expression of α-tubulin and β-tubulin.

Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-β-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-β-Tubulin (Ser-172) (lanes 3 & 4), and anti-β-Tubulin (TM1541) (lanes 5 & 6).

Western blot analysis of 250 ng/lane of a1-Tubulin unphosphorylated (lanes 1, 3, & 5) or phosphorylated at Ser-165 with PKCα (lanes 2, 4, & 6). The blots were probed with anti-α6-Tubulin (a.a. 160-169) (TP4281; lanes 1 & 2), anti-α6-Tubulin (Ser-165) (TP4131; lanes 3 & 4), and anti-α-Tubulin (C-terminus) (TM4111; lanes 5 & 6).

Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

TM1541 Wang, Q. et al. (2014) J Virol. 88(9):4853 WB: HeLa
TM4111 Gehrig, S.M. et al. (2012) Nature. 484(7394):394. WB: mouse skeletal muscle
TM1541 Meacham, CE. et al. (2011) Nature Genetics. 41:1133. WB: mouse lymphoma
TM1541 Meshki, J. et al. (2011) PLoS ONE. 6(9):e25332. WB: U3&3MG
TM1541 Campbell, S. et al. (2010) J Biol Chem. 285:4695. WB: human HeLa cells

This kit contains:

α-Tubulin (C-terminus) Mouse mAb50 μlWB, E, IP, ICC, IHCHu, Rt, Ms50
α6-Tubulin (Ser-165), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, F50
β-Tubulin Mouse mAb50 μlWB, E, ICCHu, Rt, Ms50
β-Tubulin (Ser-172), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck50
unphosphorylated βIII-Tubulin (Ser-444) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms50