Catalog # TK6560
α6-Tubulin Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, IP, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb, Ck, F |
 |
KIT SUMMARY The α-tubulin phospho-regulation kit can be used to examine phosphorylation of α-tubulin (Ser-165) relative to the total expression of α-tubulin. The kit includes rabbit polyclonal and mouse monoclonal antibodies to detect total α-tubulin, and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection. |
Western blot analysis of 250 ng/lane of a1-Tubulin unphosphorylated (lanes 1, 3, & 5) or phosphorylated at Ser-165 with PKCα (lanes 2, 4, & 6). The blots were probed with anti-α6-Tubulin (a.a. 160-169) (TP4281; lanes 1 & 2), anti-α6-Tubulin (Ser-165) (TP4131; lanes 3 & 4), and anti-α-Tubulin (C-terminus) (TM4111; lanes 5 & 6).
Western blot analysis of α-tubulin expression in human A431 (lane 1), HUVEC (lane 2), Jurkat (lane 3), mouse J774.1 (lane 4), human PC-3 (lane 5), rat PC12 (lane 6), and mouse C2C12 (lane 7). The blot was probed with anti-α-Tubulin (C-terminus) at 1:1000.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsTM4111 Gehrig, S.M. et al. (2012) Nature. 484(7394):394. WB: mouse skeletal muscle
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| α-Tubulin(C-terminus) Mouse mAb | 50 μl | WB, E, IP, ICC, IHC | Hu, Rt, Ms | 50 | |
| α6-Tubulin(a.a. 160-169) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms, Ck, F | 50 | |
| α6-Tubulin(Ser-165), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms, Ck, F | 50 | |
| Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | ||
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
