Catalog # TK6530


β-Tubulin Phospho-Regulation

Antibody Sampler Kit

$395

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in α- and β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro, and unphosphorylated Ser-444 may be an early marker for cells of neuronal lineage. Cdk1 can phosphorylate Ser-172 in β-Tubulin during mitosis and this may impair tubulin incorporation into microtubules. In α-tubulin, PKC can phosphorylate Ser-165 leading to increased cell motility in human breast cells.

References
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Mouse monoclonal and rabbit polyclonal reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

TP1691 Sitaras, N. et al. (2015) Am J Pathol. 185(2):581 IF: mouse retinal ganglion cells
TM1541 Wang, Q. et al. (2014) J Virol. 88(9):4853 WB: HeLa
TP1691 Saphieha, P. et al. (2012) Nutr Diabetes. 2:36. ICC: mouse retina cells
TM1541 Meacham, CE. et al. (2011) Nature Genetics. 41:1133. WB: mouse lymphoma
TM1541 Meshki, J. et al. (2011) PLoS ONE. 6(9):e25332. WB: U3&3MG
TM1541 Campbell, S. et al. (2010) J Biol Chem. 285:4695. WB: human HeLa cells

Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-β-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-β-Tubulin (Ser-172) (lanes 3 & 4), and anti-β-Tubulin (TM1541) (lanes 5 & 6).

Western blots of mouse brain were probed with unphosphorylated βIII-Tubulin (Ser-444) (lanes 1-3) and βIII-Tubulin (C-terminus) (lanes 4-6) polyclonal antibodies. Both antibodies were used in the presence of unphosphorylated βIII-Tublin (Ser-444) peptide (lanes 2 & 5; TX1815) and phospho-βIII-Tublin (Ser-444) peptide (lanes 3 & 6; TX1695).



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
β-Tubulin Mouse mAb50 μlWB, E, ICCHu, Rt, Ms50
β-Tubulin (a.a. 168-177) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck50
βIII-Tubulin (C-terminus) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms50
β-Tubulin (Ser-172), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck50
unphosphorylated βIII-Tubulin (Ser-444) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms50
KIT SUMMARY

The β-tubulin phospho-regulation kit can be used to examine phosphorylation of Ser-172 and unphosphorylation of Ser-444 relative to the total expression of β-tubulin or βIII-tubulin. The kit includes mouse monoclonal and rabbit polyclonal antibodies to detect all β-tubulin isoforms and a rabbit polyclonal to detect βIII-tubulin.