Catalog # TK6530

β-Tubulin Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB, ICC, IHC

Species Reactivity

Hu, Rt, Ms, Ck

$395


DATASHEET  

KIT SUMMARY

The β-tubulin phospho-regulation kit can be used to examine phosphorylation of Ser-172 and unphosphorylation of Ser-444 relative to the total expression of β-tubulin or βIII-tubulin. The kit includes mouse monoclonal and rabbit polyclonal antibodies to detect all β-tubulin isoforms and a rabbit polyclonal to detect βIII-tubulin.

Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-β-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-β-Tubulin (Ser-172) (lanes 3 & 4), and anti-β-Tubulin (TM1541) (lanes 5 & 6).

Western blots of mouse brain were probed with unphosphorylated βIII-Tubulin (Ser-444) (lanes 1-3) and βIII-Tubulin (C-terminus) (lanes 4-6) polyclonal antibodies. Both antibodies were used in the presence of unphosphorylated βIII-Tublin (Ser-444) peptide (lanes 2 & 5; TX1815) and phospho-βIII-Tublin (Ser-444) peptide (lanes 3 & 6; TX1695).



Mouse monoclonal and rabbit polyclonal reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

TP1691 Sitaras, N. et al. (2015) Am J Pathol. 185(2):581 IF: mouse retinal ganglion cells
TM1541 Wang, Q. et al. (2014) J Virol. 88(9):4853 WB: HeLa
TP1691 Saphieha, P. et al. (2012) Nutr Diabetes. 2:36. ICC: mouse retina cells
TM1541 Meacham, CE. et al. (2011) Nature Genetics. 41:1133. WB: mouse lymphoma
TM1541 Meshki, J. et al. (2011) PLoS ONE. 6(9):e25332. WB: U3&3MG
TM1541 Campbell, S. et al. (2010) J Biol Chem. 285:4695. WB: human HeLa cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
β-Tubulin Mouse mAb50 μlWB, E, ICCHu, Rt, Ms50
β-Tubulin (a.a. 168-177) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck50
βIII-Tubulin (C-terminus) Rabbit pAb50 μlWB, E, ICC, IHCHu, Rt, Ms50
β-Tubulin (Ser-172), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms, Ck50
unphosphorylated βIII-Tubulin (Ser-444) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms50