Catalog # SP4881

SMAD6 Antibody

Rabbit Polyclonal

Application Dilution
IHC1:100
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 53 kDa

DATASHEET  




$245

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 3. 5 and 8), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

 

References

Attisano, L. & Wrana, J.L. (1998) Curr Opin Cell Biol. 10:188.
Topper, J.N. et al. (1997) Natl Acad Sci. U.S.A. 94(17):9314.

Western blot analysis of SMAD6 in human Jurkat cells. The blot was probed with rabbit polyclonal SMAD6 antibody (SP4881) at 1:250.



This antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG) and amino acids 372-388 (NLEQRSESVRRTRSKIG) of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6 in multiple species.

*For more information, see UniProt Accession O43541
Rabbit polyclonal antibody purified with protein G chromatography is supplied in 100μl phosphate-buffered saline with 0.05% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects SMAD6 at 53 kDa* on SDS-PAGE immunoblots of human Jurkat cells and mouse C2C12. The antibody can used for western blot and immunohistochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY