Catalog # SP4861

SMAD4 Antibody

Rabbit Polyclonal

Application Dilution
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, Fr

MW 65 kDa

DATASHEET  




$245

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 3. 5 and 8), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

 

References

Attisano, L. & Wrana, J.L. (1998) Curr Opin Cell Biol. 10:188.
Topper, J.N. et al. (1997) Natl Acad Sci. U.S.A. 94(17):9314.

Western blot analysis SMAD4 expression in A549 cells. The blot was probed with rabbit polyclonal SMAD4 antibody (SP4861) at 1:250.



This antibody was developed against synthetic peptides corresponding to amino acids 186-199 (PSNRASTETYSTPA) and amino acids 509-523(WGPDYPRQSIKETPC) of human SMAD4 (GenBank Accession No. NP_005350.1). These peptide sequences are specific to SMAD4 and have high homology to SMAD4 in multiple species.

*For more information, see UniProt Accession Q13485
Rabbit polyclonal antibody purified with protein G chromatography is supplied in 100μl phosphate-buffered saline with 0.2% gelatin and 0.05% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects SMAD4 at 65 kDa* on SDS-PAGE immunoblots of human A549 cells and in enterochromaffin cells treated with TGFβ.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY