Catalog # SM3591

Smac/DIABLO (C-terminal region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 23 kDa

DATASHEET  




$245

X linked inhibitor of apoptosis (XIAP) is a member of the inhibitor of apoptosis (IAP) family of proteins, and is involved in regulating programmed cell death by caspase inhibition. SMAC/DIABLO (second mitochondria derived activator of caspase/direct IAP binding protein with low PI) is expressed in a variety of tissues, and has been identified as a negative regulator of XIAP. A functionally active nine-residue peptide derived from the N terminus of Smac/DIABLO interacts with the baculovirus IAP repeat 3 domain of XIAP. Smac/DIABLO promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to IAPs and removing their inhibitory activity. Smac/DIABLO is normally a mitochondrial protein but is released into the cytosol when cells undergo apoptosis.

 

References

Du, C. et al. (2000) Cell 102:33.
Liu, Z. et al. (2000) Nature 408:1004.
Verhagen, A.M. et al. (2000) Cell 102:43.

Western blot analysis of Smac/DIABLO expression in A431 cell lysate. The blots were probed with mouse monoclonal Smac/DIABLO (C-terminal region) at 1:500 (lane 1) or 1:2000 (lane 2).

Immunocytochemical labeling of Smac/Diablo in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal Smac/Diablo (C-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.



Clone M359 was generated from a recombinant protein that included amino acid residues within the C-terminal region of human Smac/DIABLO. This sequence has high homology with similar regions in rat and mouse Smac/DIABLO.

*For more information, see UniProt Accession Q9NR28
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 23 kDa* band corresponding to the molecular weight of Smac/DIABLO on SDS-PAGE immunoblots of A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY