Catalog # RS3241


Anti-Rabbit Ig Light-Chain Specific, unconjugated

Mouse Monoclonal

Application Dilution
ELISA20 ng/lane

Size 100 μl

Species Reactivity Rb

Isotype IgG1

$55

An array of antibody-based immunoassays require the use of secondary reagents that detect mouse, rabbit, rat, or goat primary antibodies. These secondary reagents are usually conjugated to a detection reagent, such as horse radish peroxidase (HRP). HRP is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or the release of light as a byproduct of the reaction. HRP functions optimally at a near-neutral pH and can be inhibited by cyanides, sulfides and azides. In addition, its high turnover rate, good stability, low cost and wide availability of substrates makes HRP the enzyme of choice for most applications. Mouse anti-Rabbit Ig Light-Chain Specific:HRP can be used for chemiluminescent readout in western blot, colorimetric readout in ELISA, and precipitation reactions useful in immunocytochemistry and immunohistochemistry. Mouse anti-Rabbit Ig Light-Chain Specific unconjugated is also available for ELISA sandwich assays and for antibody blocking experiments where no detection reagent is required.

 

References
Mattson, D.L. & Bellehumeur, T.G. (1996). Anal. Biochem. 240:306.
The clone 5A6-1D10 mouse monoclonal secondary reagent reacts with the light chains, but not heavy chains from rabbit immunoglobulins, and has minimal cross-reactivity with bovine, goat, horse, human, mouse, rat, and sheep immunoglobulins.

Mouse monoclonal antibody purified with protein A chromatography is supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody reacts with the light chains of rabbit immunoglobulins in various antibody applications that require unconjugated anti-rabbit reactivity. In immunoprecipitation reactions, the antibody detects 20-30 kDa bands corresponding to the light chains of rabbit immunoglobulins.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY