The retinoblastoma gene product (Rb) is a tumor suppressor protein that acts as cell cycle regulator and has roles in apoptosis. During apoptosis, Rb is subjected to cleavage by caspase-3 like protease. Caspase-3 cleaves Rb into 48 and 68kDa fragments. Cleavage of Rb would make it functionally inactive, causing release of E2F-1. The E2F-1 then may promote apoptosis. RM4931 mouse monoclonal antibody recognizes cleaved Rb (deltaRb-p70), but does not recognize full-length Rb. In camptothecin treated HL60, an ~68 kDa band is observed. deltaRb-p70 exists in vivo as an N-terminally truncated form of Rb. deltaRb-p70 is thought to be the product of alternative translation and is expressed in primary myeloid cells in fetal liver, bone marrow and spleen. DRb-p70 is also specifically expressed in tumor-associated macrophages (TAMs). TAMs are an important targets of cancer therapy. deltaRb-p70 is a marker of TAMs, and maybe useful for identifying tumors with macrophage infiltration.
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*For more information, see UniProt Accession P06400
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains: