Catalog # RM3351


RasGAP

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 120 kDa

Isotype IgG1

$245

The Ras family (K-Ras, H-Ras, and N-Ras) includes 21 kDa guanine-nucleotide binding proteins that cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAP kinase pathway. Both GTPase-activating proteins (GAPs), such as p120 Ras-GAP, and guanine-nucleotide exchange factors (GEFs) regulate the activity of Ras proteins in many cell signaling pathways. Mutations in Ras are found in several cancers, and the most common oncogenic mutation is Gly12 to Asp (G12D), which prevents Ras inactivation.

 

References

Ma, J. & Karplus, M. (1997) J. Mol. Biol. 274:114.
Boguski, M.S. & McCormick, F. (1993) Nature 366:643.
Buday, L. & Downward, J. (1993) Cell 73:611.
Huang, D.C. et al. (1993) Mol. Cell Biol. 13:2420.

Clone (M335) was generated from a recombinant sequence in the central region of human RasGAP. This sequence has high homology to mouse and rat RasGAP.

*For more information, see UniProt Accession P20936
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 120 kDa* protein corresponding to the molecular mass of RasGAP on SDS-PAGE immunoblots of adult mouse brain, human A431, Jurkat, and SYF fibroblasts.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of mouse brain (lane 1), human A431 (lane 2), Jurkat (lane 3), and SYF fibroblast lysates. The blot was probed with mouse monoclonal anti-RasGAP antibody at 1:1000.

Immunocytochemical labeling of RasGap in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal RasGap (RM3351) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.



This kit contains:

KIT SUMMARY