Catalog # RM2761

ROCK-II (Central region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 165 kDa

Isotype IgG1

DATASHEET  




$245

Rho-associated kinases (ROCKs) are a family of serine/threonine kinases that have important roles in Rho-mediated signaling. The structure of ROCKs (ROCK-I/ROKβ and ROCK-II/ROKα) includes an N-terminal catalytic domain, a coiled-coil region, and a C-terminal pleckstrin homology domain. The C-terminal region negatively regulates kinase activity, and this inhibitory regulation is controlled through C-terminal cleavage. Caspase-3-induced cleavage of ROCK-I and direct cleavage of ROCK-II by granzyme B activate ROCK. This leads to inhibition of myosin phosphatase and phosphorylation of myosin light chain, which causes myosin II-mediated changes in cytoskeletal dynamics. This pathway has been implicated as a downstream effector of Rho-mediated changes in cytokinesis, cell motility, apoptosis and muscle contraction.

 

References

Sebbagh, M. et al. (2005) J. Exp. Med. 201:465.
Lee, J.H. et al. (2004) J. Cell. Biol. 167:327.
Nakagawa, O. et al. (1996) FEBS Lett. 392:189.

Western blot analysis of human Jurkat cells (lane 1), rat PC12 cells (lane 2), mouse brain tissue (lane 3), and mouse SYF fibroblasts (lane 4). The blot was probed with mouse monoclonal anti-ROCK-II (Central region) antibody at 1:1000 dilution (lanes 1-4).



Clone (M276) was generated from amino acids in the central region of human ROCK-II. This sequence has high homology to rat and mouse ROCK-II, and has 50% homology to ROCK-I.

*For more information, see UniProt Accession Q28021
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 165 kDa* band corresponding to ROCK-II in mouse kidney, heart, and brain tissues, as well as in rat PC12, mouse SYF, and human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY