Catalog # RM2741

ROCK-I (C-terminal), cleavage-specific Antibody

Mouse Monoclonal

Application Dilution
ELISA1:500
WB1:250

Size 200 μl

Species Reactivity Hu, Rt, Ms

MW 31 kDa

Isotype IgG1

DATASHEET  




$295

Rho-associated kinases (ROCKs) are a family of serine/threonine kinases that have important roles in Rho-mediated signaling. The structure of ROCKs (ROCK-I/ROKβ and ROCK-II/ROKα) includes an N-terminal catalytic domain, a coiled-coil region, and a C-terminal pleckstrin homology domain. The C-terminal region negatively regulates kinase activity, and this inhibitory regulation is controlled through C-terminal cleavage. Caspase-3-induced cleavage of ROCK-I and direct cleavage of ROCK-II by granzyme B activate ROCK. This leads to inhibition of myosin phosphatase and phosphorylation of myosin light chain, which causes myosin II-mediated changes in cytoskeletal dynamics. This pathway has been implicated as a downstream effector of Rho-mediated changes in cytokinesis, cell motility, apoptosis and muscle contraction.

 

References

Sebbagh, M. et al. (2005) J. Exp. Med. 201:465.
Lee, J.H. et al. (2004) J. Cell. Biol. 167:327.
Nakagawa, O. et al. (1996) FEBS Lett. 392:189.

Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with camptothecin (lanes 2 & 4). The blots were probed with anti-ROCK-I (RM2721) (lanes 1 & 2) and anti-ROCK-I (C-terminal) cleavage-specific (RM2741) (lanes 3 & 4) antibodies.



Clone (154C1465) was generated from a.a. 1114-1126 adjacent to the caspase cleavage site in human ROCK-I. This sequence has high homology to rat and mouse ROCK-I, and has significant homology to the conserved region in ROCK-II.

*For more information, see UniProt Accession Q13464
Mouse monoclonal purified with protein G chromatography is supplied in 200μl phosphate-buffered saline with 0.2% gelatin and 0.05% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 31 kDa* band corresponding to the C-terminal cleavage fragment of ROCK-I on SDS-PAGE immunoblots of human Jurkat cells treated with camptothecin.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY