Catalog # RM2721
ROCK-I
Mouse Monoclonal
| ELISA | 1:1000 |
| WB | 1:500 |
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 160 kDa
Isotype IgG1
Rho-associated kinases (ROCKs) are a family of serine/threonine kinases that have important roles in Rho-mediated signaling. The structure of ROCKs (ROCK-I/ROKβ and ROCK-II/ROKα) includes an N-terminal catalytic domain, a coiled-coil region, and a C-terminal pleckstrin homology domain. The C-terminal region negatively regulates kinase activity, and this inhibitory regulation is controlled through C-terminal cleavage. Caspase-3-induced cleavage of ROCK-I and direct cleavage of ROCK-II by granzyme B activate ROCK. This leads to inhibition of myosin phosphatase and phosphorylation of myosin light chain, which causes myosin II-mediated changes in cytoskeletal dynamics. This pathway has been implicated as a downstream effector of Rho-mediated changes in cytokinesis, cell motility, apoptosis and muscle contraction.
References
Sebbagh, M. et al. (2005) J. Exp. Med. 201:465.
Lee, J.H. et al. (2004) J. Cell. Biol. 167:327.
Nakagawa, O. et al. (1996) FEBS Lett. 392:189.
*For more information, see UniProt Accession Q13464
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with camptothecin (lanes 2 & 4). The blots were probed with anti-ROCK-I (RM2721) (lanes 1 & 2) and anti-ROCK-I (C-terminal) cleavage-specific (RM2741) (lanes 3 & 4) antibodies.
This kit contains:
