Catalog # RM2721

ROCK-I Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:1000
WB	1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 160 kDa

Isotype IgG1

Rho-associated kinases (ROCKs) are a family of serine/threonine kinases that have important roles in Rho-mediated signaling. The structure of ROCKs (ROCK-I/ROKβ and ROCK-II/ROKα) includes an N-terminal catalytic domain, a coiled-coil region, and a C-terminal pleckstrin homology domain. The C-terminal region negatively regulates kinase activity, and this inhibitory regulation is controlled through C-terminal cleavage. Caspase-3-induced cleavage of ROCK-I and direct cleavage of ROCK-II by granzyme B activate ROCK. This leads to inhibition of myosin phosphatase and phosphorylation of myosin light chain, which causes myosin II-mediated changes in cytoskeletal dynamics. This pathway has been implicated as a downstream effector of Rho-mediated changes in cytokinesis, cell motility, apoptosis and muscle contraction.

References

Sebbagh, M. et al. (2005) J. Exp. Med. 201:465.
Lee, J.H. et al. (2004) J. Cell. Biol. 167:327.
Nakagawa, O. et al. (1996) FEBS Lett. 392:189.

Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with camptothecin (lanes 2 & 4). The blots were probed with anti-ROCK-I (RM2721) (lanes 1 & 2) and anti-ROCK-I (C-terminal) cleavage-specific (RM2741) (lanes 3 & 4) antibodies.

Clone (M272) was generated from a recombinant protein that included amino acids near the RhoA binding reigon of human ROCK-I. This sequence has high homology to rat and mouse ROCK-I, and has 50% homology to ROCK-II.

*For more information, see UniProt Accession Q13464

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 160 kDa* band corresponding to ROCK-I in mouse kidney, rat PC12 cells, and human Jurkat cells. In addition, the antibody detects the 130 kDa caspase-cleavage product in Jurkat cells treated with camptothecin.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: