Catalog # PM4851
p73/δNp73 (N-terminus) Antibody
Mouse Monoclonal
| ICC | 1:50 |
| IHC | 1:50 |
| WB | 1:500 |
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 73 kDa
Isotype IgG1
p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 have been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct C-terminal structures suggesting that the C-terminal region of p73 may be important for its function. A p73 variant has been identified that lacks an N-terminal transactivation domain, called δNp73. The δNp73 message is transcribed from an alternative promoter located in intron 3.
References
Levrero M. et al. (2000) J Cell Sci. 113 (10):1661.
Ueda Y. et al. (1999) Oncogene. 18:4993.
Kaghad M. et al. (1997) Cell. 90:809.
Western blot analysis for δNp73 using anti-δNp73 (N-terminus) antibody PM4851 at 1:500 in A) a cell line transfected with δNp73 cDNA, B) HeLa, and C) NIH 3T3 cell lysate.
*For more information, see UniProt Accession O15350
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
