Catalog # PM4851

p73/δNp73 (N-terminus) Antibody

Mouse Monoclonal

Application Dilution
ICC1:50
IHC1:50
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 73 kDa

Isotype IgG1

DATASHEET  




$245

p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 have been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct C-terminal structures suggesting that the C-terminal region of p73 may be important for its function. A p73 variant has been identified that lacks an N-terminal transactivation domain, called δNp73. The δNp73 message is transcribed from an alternative promoter located in intron 3.

 

References
Sayan A.E. et al. (2008) Oncogene. 27:4363.
Levrero M. et al. (2000) J Cell Sci. 113 (10):1661.
Ueda Y. et al. (1999) Oncogene. 18:4993.
Kaghad M. et al. (1997) Cell. 90:809.

Western blot analysis for δNp73 using anti-δNp73 (N-terminus) antibody PM4851 at 1:500 in A) a cell line transfected with δNp73 cDNA, B) HeLa, and C) NIH 3T3 cell lysate.



Clone 38C674.2 was generated from amino acids 2 to 13 in human δNp73. This sequence has high homology in mouse and rat δNp73.

*For more information, see UniProt Accession O15350
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 0.05% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects δNp73 on SDS-PAGE immunoblots of δNp73-transfected cells, as well as human HeLa cells and mouse NIH-3T3 cells. The antibody works for western blot, immunocytochemistry, and chromatin immunoprecipitation assays.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY