Catalog # PM4831


p73 (N-terminal region)

Mouse Monoclonal

Application Dilution
ICC1:50
IHC1:50
IP1:50
WB1:250

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 73 kDa

Isotype IgG1

$245

p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 have been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct C-terminal structures suggesting that the C-terminal region of p73 may be important for its function. A p73 variant has been identified that lacks an N-terminal transactivation domain, called δNp73. The δNp73 message is transcribed from an alternative promoter located in intron 3.

 

References
Sayan A.E. et al. (2008) Oncogene. 27:4363.
Levrero M. et al. (2000) J Cell Sci. 113 (10):1661.
Ueda Y. et al. (1999) Oncogene. 18:4993.
Kaghad M. et al. (1997) Cell. 90:809.
Clone 5B429 was generated from full-length human p73, and the antibody reacts with an epitope in the N-terminal region of human p73. The antibody does not detect δNp73 and does not cross react with p53.

*For more information, see UniProt Accession O15350
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 0.05% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects p73 at 73 kDa* on SDS-PAGE immunoblots of p73-transfected cells, as well as p73 in human HeLa cells, and many other human, mouse, and rat cell types and tissues.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate using anti-p73 (N-terminal region) PM4831 at 1:250.



This kit contains:

KIT SUMMARY