Catalog # PM4741

PARP (cleavage specific) Antibody

Mouse Monoclonal

Application Dilution
FC1:500
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 89 kDa

Isotype IgG2b

DATASHEET  




$245

The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.

 

References
Smulson, M.E. et al. (1998) Cancer Res. 58(16):3495.
Tewari, M. et al. (1995) Cell. 81:801.
Kaufmann, S.H. et al. (1993) Cancer Res. 53:3976.

Western blot analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points. The blots were probed with anti-Parp (cleavage specific) at 1:250. The band corresponding to cleaved PARP is 89 kDa and is induced after 1 to 4 hrs of staurosporine treatment.



Clone 194C1439 was generated from a synthetic peptide corresponding to amino acids around the 214/215-cleavage site in human PARP. This sequence is well conserved in rat and mouse PARP, but the antibody has not been tested for these species reactivities.

*For more information, see UniProt Accession P09874
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 0.05% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects an 89 kDa* protein corresponding to the molecular mass of the cleaved fragment of PARP induced by staurosporine treatment of Jurkat cells. The antibody can be used to identify caspase-3 activity during apoptosis.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY