Catalog # PM1101


PKC (α,β2,γ)

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 82 kDa

Isotype IgG1

$245

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCα and PKCβI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.

 

References

Kawakami et al. (2003) PNAS. USA 100:9470-9475.
Nishizuka, Y. (1988) Nature 334:661.

Clone (M110) was generated from a recombinant human PKCγ that included amino acids residues 499-697.

*For more information, see UniProt Accession P05129
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects 80-82kDa* proteins corresponding to the molecular mass of PKCα, PKCβ, and PKCγ on SDS-PAGE immunoblots of neonatal rat brain lysates. Similar results were observed in human and mouse lysates. Immunoprecipitation experiments with various PKC isoforms demonstrated this antibody detects PKCα, PKCβ2, and PKCγ, but does not detect PKCβ2 isoform. The antibody also detects these PKC isoforms in chick DRG neurons.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of PKC isoforms in neonatal rat brain lysate. The rat brain blot was probed with anti-PKC (α,β,γ) at decreasing dilutions:

Immunocytochemical labeling of PKC relative to F-actin in chick DRG neurons. The cells were labeled with mouse monoclonal PKC (α,β,γ) antibody (PM1101), then detected using appropriate secondary antibody (Red). This labeling is compared to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).



This kit contains:

KIT SUMMARY