Catalog # NM4691


NFκB (p65)

Mouse Monoclonal

Application Dilution
FC1:500
IHC1:50
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 65 kDa

Isotype IgG1

$245

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.

 

References
Waris et al. (2003) J Biol Chem 278(42):40778.
Bui, N.T. et al. (2001) J Cell Biol 152(4):753.
Finco, T.S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:11884.
Clone 112A1021 was generated from a synthetic peptide corresponding to amino acid 526-539 from human NFκB. This sequence is well conserved in rat and mouse NFκB.

*For more information, see UniProt Accession Q04206
Mouse monoclonal purified with protein G chromatography is supplied in 100μl phosphate-buffered saline containing 0.05% BSA and 0.05% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects NFκB at 65 kDa* on SDS-PAGE immunoblots of Ramos, Daudi, HeLa, and mouse NIH3T3 cells. The antibody can be used for western blot, immunohistochemistry, and flow cytometry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of NFκB expression in Ramos (lane 1), Daudi (lane 2), HeLa (lane 3), and mouse NIH3T3 (lane 4). The blot was probed with mouse monoclonal NFκB (NM4691) at 1:250 dilution.

Immunohistochemical analysis of NFκB in formalin-fixed, paraffin-embedded human tonsil using mouse monoclonal NFκB antibody (NM4691).



This kit contains:

KIT SUMMARY