Catalog # NM3911

Neuropilin-2 (MAM Domain) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 130 kDa

Isotype IgG1

DATASHEET  




$245

Neuropilins are transmembrane proteins that contain two CUB domains (a1 and a2), two coagulation factor-like domains (b1 and b2), and a MAM domain in the extracellular region. These proteins have short cytoplasmic domains that include a PDZ-binding motif. The neuropilin (NRP) family includes NRP-1, NRP-2A, and NRP-2B. NRP-1 and NRP-2 can form a heterodimer. NRP-1 binds with high affinity to Semaphorin 3A, while NRP-2 binds with high affinity to Semaphorin 3F. Both NRP-1 and NRP-2 have been implicated in VEGFR and Plexin receptor function, and both neuropilins may be involved in axon guidance, angiogenesis, and tumor progression.

 

References
Nakamura, F. & Goshima, Y. (2002) Adv Exp Med Biol. 515:55.
He, Z. & Tessier-Lavigne, M. (1997) Cell 90:739.
Kolodkin, A.L. et al. (1997) Cell 90:753.

Western blot analysis of neuropilin-2 (NRP-2) expression in neonatal rat brain. The blot was probed with mouse monoclonal anti-NRP-2 (MAM Domain) at 1:250.



Clone M391 was generated from Neuropilin-2 recombinant protein containing amino acids in the MAM domain region of rat neuropilin-2. This sequence is highly conserved in human and mouse neuropilin-2, and has low homology to neuropilin-1.

*For more information, see UniProt Accession O35276
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of neuropilin-2 on SDS-PAGE immunoblots of rat brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY