Catalog # NM3641


Nogo A (Central region)

Mouse Monoclonal

Application Dilution
ELISA1:1000
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 180 kDa

Isotype IgG1

$245

After central nervous system (CNS) injury, there is minimal regeneration of axonal projections through the injured region, which compromises functional recovery from these injuries. Various inhibitory molecules found in the myelin of axons in the CNS may limit regeneration through activation of inhibitory cell signaling pathways. Nogo was identified in myelin extracts using the monoclonal antibody IN-1, an antibody that allows modest axon regeneration after spinal cord injury. Nogo is expressed as three different proteins, Nogo-A, -B, and -C, which are members of the Reticulon family of ER anchoring proteins. Nogo-A is the full length protein, while Nogo-B contains 172 amino acids of the N-terminus and 188 amino acids of the C-terminus of Nogo-A, while Nogo-C contains only the 188 amino acid C-terminus of Nogo-A. These splice variants are all found in optic nerve, spinal cord, and cerebral cortex, but differ in expression in other neuronal and non-neuronal tissues. Thus, Nogo-A may be an important myelin-associated protein limiting axon regeneration after CNS injury.

 

References
Chen, M.S. et al. (2000) Science 403:434.
GrandPre, T. et al.( 2000) Nature 403:439.
Tessier-Lavigne, M. & Goodman, C.S. (2000) Science 287:813.
Clone M364 was generated from a sequence corresponding to amino acids in the central region of rat Nogo A. This sequence has high homology to human and mouse Nogo A.

*For more information, see UniProt Accession Q9JK11
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 180 kDa* protein corresponding to the molecular mass of Nogo A on SDS-PAGE immunoblots of postnatal day 1 rat brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of postnatal day 1 rat brain lysate. The blot was probed with mouse monoclonal anti-Nogo A (Central region) antibody at 1:500.



This kit contains:

KIT SUMMARY