After central nervous system (CNS) injury, there is minimal regeneration of axonal projections through the injured region, which compromises functional recovery from these injuries. Various inhibitory molecules found in the myelin of axons in the CNS may limit regeneration through activation of inhibitory cell signaling pathways. Nogo was identified in myelin extracts using the monoclonal antibody IN-1, an antibody that allows modest axon regeneration after spinal cord injury. Nogo is expressed as three different proteins, Nogo-A, -B, and -C, which are members of the Reticulon family of ER anchoring proteins. Nogo-A is the full length protein, while Nogo-B contains 172 amino acids of the N-terminus and 188 amino acids of the C-terminus of Nogo-A, while Nogo-C contains only the 188 amino acid C-terminus of Nogo-A. These splice variants are all found in optic nerve, spinal cord, and cerebral cortex, but differ in expression in other neuronal and non-neuronal tissues. Thus, Nogo-A may be an important myelin-associated protein limiting axon regeneration after CNS injury.
GrandPre, T. et al.( 2000) Nature 403:439.
Tessier-Lavigne, M. & Goodman, C.S. (2000) Science 287:813.
*For more information, see UniProt Accession Q9JK11
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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