nNOS Phospho-Regulation Antibody Kit

Nitric oxide (NO) has a broad range of biological activities and is implicated in many cell signaling pathways. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are fused enzymes that include a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). The NOS family includes two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designated ecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is induced by microbial products in various cell types. Phosphorylation of NOS enzymes may regualte their activity. For example, Src kinase-induced phosphorylation of Tyr-1055 in iNOS reduces proteasomal degradation of iNOS, while shear stress induced phosphorylation of Tyr-657 in eNOS attenuates enzyme activity. Both of these tyrosine sites are conserved in nNOS, and may have important roles for regulate neuronal NOS activity.

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