Catalog # NK6550
nNOS Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb, Ck |
 |
KIT SUMMARY The nNOS phospho-regulation kit can be used to examine phosphorylation of nNOS (Tyr-895)/eNOS (Tyr-657) and nNOS (Tyr-1326)/iNOS (Tyr-1055) conserved sites relative to the total expression of nNOS. The kit includes rabbit polyclonal and mouse monoclonal antibodies to detect total nNOS, and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection. |
Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).
Labeling of nNOS phosphorylation in rat PC12 cells. The cells were probed with mouse monoclonal (mAb) nNOS (NM4011), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using secondary antibody conjugated to DyLight® 594.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsNM4011 Stasko, S. et al. (2013) J Appl Physiol. 114(11):1629. WB: mouse diaphragm, normal, deficient
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| eNOS (Tyr-657)/nNOS (Tyr-895), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 140 | |
| iNOS (Tyr-1055)[conserved site], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck | 130 | |
| nNOS (C-terminal region) Mouse mAb | 50 μl | WB, E, ICC, IHC | Hu, Rt, Ms | 155 | |
| nNOS (C-terminal regiom) Rabbit pAb | 50 μl | WB, E, ICC, IHC | Hu, Rt, Ms | 155 | |
| Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | ||
| Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
