Catalog # MM3361

MKK4 (N-terminal region) Antibody

Mouse Monoclonal

Application / Dilution

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 44 kDa

Isotype IgG1



The stress-activated protein kinases (SAPK) or Jun-amino-terminal kinases (JNK) are potently activated by stressors such as UV and gamma radiation. Similar to other MAP Kinases, the core signaling unit is composed of a MAPKKK, usually MEKK1-4 or a mixed lineage kinase (MLK), which phosphorylate and activate MKK4-7, leading to dual phosphorylation and activation of JNK kinases. The MKK family member MKK4 (SEK1/JNKK1) is activated downstream of Rho-GTPases by MEKK or MLK phosphorylation of Ser-257 and Thr-261, while Akt phosphorylation of Ser-80 inhibits MKK4 and suppresses stress-activated signal transduction.


Park, H. et al. (2002) J. Biol. Chem. 277:2573.
Kyriakis, J.M. et al. (1994) Nature 369:156.
Sanchez, I. et al. (1994) Nature 372:794.
Yan, M. et al. (1994) Nature 372:798.

Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-MKK4 (N-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3) or 1:2000 (lane 4).

Clone (M336) was generated from a sequence corresponding to amino acids in the N-terminal region of the human MKK4. This sequence has high homology to rat and mouse MKK4.

*For more information, see UniProt Accession P45985
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 44 kDa* protein corresponding to the molecular mass of MKK4 on SDS-PAGE immunoblots of adult mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: