Catalog # LM5971


Laminin γ1 subunit (Domain IV)

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 220 kDa

Isotype IgG1

$295

The basal lamina contains Collagen Type IV, proteoglycans, and glycoproteins. Laminin is a high molecular weight (850 kDa) oligomer, consisting of three different chains (α, β, and γ) joined by disulfide bonds. The structure of laminins include two helical domains (I & II) at the COOH-terminal, a laminin IV domain, multiple EGF-like repeats, and a laminin globular domain (G), as well as an N-terminal domain VI. Domains IV and VI are the binding sites for collagen and heparan sulfate, respectively. Several isoforms have been identified for the genes of each chain. Laminin γ1 (laminin B2) contains 14 glycosylation sites and 12 cysteine repeat domains. The expression of the Laminin subunits is found in the basal lamina of tissues. Here, the protein interacts with other extracellular matrix components to mediate cell attachment, migration and organization during embryonic development.

 

References
Wewer, U.M. (1992) Lab Invest. 66(3):378.
Pikkarainen, T. et al. (1988) J Biol Chem. 263(14):6751.
Clone (M597) was generated from a recombinant protein containing amino acids in domain IV of human laminin γ1 subunit. This sequence is highly conserved in mouse and rat laminin γ1 subunits.

*For more information, see UniProt Accession P11047
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M597 was purified using Protein A chromatography. The laminin γ1 antibody detects a 220 kDa* protein corresponding to endogenous laminin γ1 expressed in human MDA-MB-231 and HeLa cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot image of human MDA-MB-231 cells (lanes 1-3). The blot was probed with mouse monoclonal anti-Laminin γ1 subunit (LM5971) at 1:500 (lane 1), 1:1000 (lane 2), and 1:2000 (lane 3).

Immunocytochemical labeling of laminin γ1 subunit in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-laminin γ1 subunit (LM5971). The antibody was detected using goat anti-mouse DyLight® 594.



This kit contains:

KIT SUMMARY