Microtubule-based kinesin superfamily motors move along microtubles during an array of cellular events, such as intracellular transport, mitosis, cytoskeletal rearrangement, and signal transduction. KIF11 (Eg5/Trip5/KNSL1/HKSP) is a Kinesin-5 family member with a conserved N-terminal catalytic motor domain followed by a neck linker leading to a coiled-coil stalk. This motor protein walks processively toward the plus-end of microtubules. KIF11 forms a homotetramer that includes a dimer in a parallel coiled-coil form complexed with a second dimer in an anti-parallel coiled-coil tetramer with four motor domains. This allows KIF11 to crosslink two adjacent microtubules such that each dimeric motor unit interacts with a single protofilament on each microtubule. KIF11 plays an essential role in the proper separation of spindle poles and the correct formation of bipolar mitotic spindle during mitosis. Inhibition of this protein results in cells apoptosis followed by mitotic arrest and the formation of characteristic monoaster spindles. Thus, KIF11 is an attractive anticancer therapeutic target.
Valentine M.T. & Gilbert S.P. (2007) Curr Opin Cell Biol. 19(1):75.
*For more information, see UniProt Accession P52732
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains: