Catalog # KM2931


KIF11/Eg5 (Central region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 120 kDa

Isotype IgG1

$245

Microtubule-based kinesin superfamily motors move along microtubles during an array of cellular events, such as intracellular transport, mitosis, cytoskeletal rearrangement, and signal transduction. KIF11 (Eg5/Trip5/KNSL1/HKSP) is a Kinesin-5 family member with a conserved N-terminal catalytic motor domain followed by a neck linker leading to a coiled-coil stalk. This motor protein walks processively toward the plus-end of microtubules. KIF11 forms a homotetramer that includes a dimer in a parallel coiled-coil form complexed with a second dimer in an anti-parallel coiled-coil tetramer with four motor domains. This allows KIF11 to crosslink two adjacent microtubules such that each dimeric motor unit interacts with a single protofilament on each microtubule. KIF11 plays an essential role in the proper separation of spindle poles and the correct formation of bipolar mitotic spindle during mitosis. Inhibition of this protein results in cells apoptosis followed by mitotic arrest and the formation of characteristic monoaster spindles. Thus, KIF11 is an attractive anticancer therapeutic target.

 

References
Zhang Y. & Xu W. (2008). Anticancer Agents Med Chem. 8(6):698.
Valentine M.T. & Gilbert S.P. (2007) Curr Opin Cell Biol. 19(1):75.
Clone (M293) was generated from a sequence corresponding to amino acids near the coiled-coil region of human KIF11. This sequence has high homology to rat and mouse KIF11.

*For more information, see UniProt Accession P52732
Mouse monoclonal, protein G purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 120 kDa* protein corresponding to the molecular mass of KIF11 on SDS-PAGE immunoblots of human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-KIF11 (Central region) antibody at 1:250 (lane 1) and 1:1000 (lane 2).



This kit contains:

KIT SUMMARY