Catalog # JM3381


Jak1 (Central region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
IP1:50
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 130 kDa

Isotype IgG1

$245

The Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors. Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for several signaling proteins, such as Stats, Shc, and focal adhesion kinase (FAK). Thus, Jak kinases are important downstream mediators of cytokine signaling pathways.

 

References
Leonard, W.J. & O'Shea, J.J. (1998) Annu. Rev. Immunol. 16:293.
Darnell, J.E. (1997) Science 277:1630.
Clone (M338) was generated from a sequence corresponding to amino acids in the central region of mouse Jak1. This sequence has high homology to human and rat Jak1.

*For more information, see UniProt Accession P52332
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 130 kDa* protein corresponding to the molecular mass of Jak1 on SDS-PAGE immunoblots of adult mouse brain and human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-Jak1 (Central region) antibody at 1:250 (lane 1) or 1:1000 (lane 2).



This kit contains:

KIT SUMMARY