Catalog # IM5951


Integrin α5 (Extracellular region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu

MW 150 kDa

Isotype IgG2a

$245

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. Integrin α5 and integrin β1 form a receptor for fibronectin and fibrinogen. This receptor is important for vascular development. Both integrin subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. Integrin α5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements.

 

References
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Clone M595 was generated from a recombinant protein containing amino acid residues in the extracellular region of human Integrin α5. This sequence has low homology to other integrin family members.

*For more information, see UniProt Accession P08648
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M595 was purified using Protein A chromatography. The antibody detects a 150 kDa* protein corresponding to the molecular mass of Integrin α5 on SDS-PAGE immunoblots of human HeLa and A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of human HeLa cell lysates. The blot was probed with mouse monoclonal anti-Integrin α5 (IM5951) at a dilution of 1:500 (lane 1) and 1:2000 (lane 2).



This kit contains:

KIT SUMMARY