Catalog # IM5941


Integrin αL (Extracellular region)

Mouse Monoclonal

Application Dilution
ELISA1:1000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 180 kDa

Isotype IgG1

$245

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members. Integrin β2/αL is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

 

References
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Clone M594 was generated from a recombinant protein containing amino acid residues in the extracellular region of human Integrin αL. This sequence has low homology to other integrin family members.

*For more information, see UniProt Accession P20701
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M594 was purified using Protein A chromatography. The antibody detects a 180 kDa* protein corresponding to the molecular mass of Integrin αL on SDS-PAGE immunoblots of human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of human Jurkat whole cell lysate. The blot was probed with mouse monoclonal anti-integrin αL (IM5941) at a dilution of 1:500 (lane 1), 1:1000 (lane 2), 1:2000 (lane 3) and 1:4000 (lane 4).



This kit contains:

KIT SUMMARY