Catalog # IM5831


Integrin α2 (Extracellular region)

Mouse Monoclonal

Application Dilution
ELISA1:1000
WB1:500
ICC1:50

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 150 kDa

Isotype IgG2a

$295

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β3 subunit associates with integrin α2 in platelets where this glycoprotein complex acts as a fibrinogen receptor and mediates platelet aggregation. In endothelial cells, integrin β3 complexes with the integrin αV subunit to form the vitronectin receptor. This receptor mediates endothelial cell adhesion to vitronectin, fibrinogen, von Willebrand factor, thrombospondin, laminin, and fibronectin.

 

References
Iwamoto, D, Calderwood, D.(2015) Curr Opin Cell Biol. 36:41.
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Clone M583 was generated from a recombinant protein containing amino acid residues in the extracellular region of human Integrin α2. This sequence has high homology with rat and mouse Integrin α2, and has low homology to other integrin family members.

*For more information, see UniProt Accession P17301
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 150 kDa* protein corresponding to the molecular mass of Integrin α2 on SDS-PAGE immunoblots of human MDA-MB-231 and A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of human MDA-MB-231 (lane 1) and A431 (lane 2) cells. The blot was probed with mouse monoclonal anti-Integrin α2 (IM5831) at 1:1000 dilution.

Immunocytochemical labeling of Integrin α2 in aldehyde-fixed and NP-40-permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Integrin α2 (IM5831) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.



This kit contains:

KIT SUMMARY