Catalog # IM4811

IKKε/TBK1 Antibody

Mouse Monoclonal

Application Dilution
FC1:500
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 80 kDa

Isotype IgG2a

DATASHEET  




$245

NF-κB (nuclear factor κB) is sequestered in the cytoplasm by IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least four proteins, IKKα, IKKβ, IKKγ, and IKKε. IKKα and IKKβ are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKKβ, not IKKα, is the target of proinflammatory stimuli. Targeted disruption of IKKβ gene in mice also results in extensive liver damage from apotopsis and death during embryo development. IKKε has significant homology with IKKα and IKKβ. LPS increases IKKε mRNA level in mouse macrophages, and overexpression of wild type IKKε leads to phosphorylation of Ser-32 and Ser-36 of IκBα.

 

References
Hu, Y. et al. (1999) Science 284:316.
Takada, K. et al. (1999) Science 284:313.
Delhase, M. et al. (1999) Science 284:309.
Regnier, C.H. et al. (1997) Cell. 90:373.

Western blot analysis of IKKε in human Jurkat cells. The blot was probed with mouse monoclonal IKKε (IM4811) at a dilution of 1:250.



Clone 72B587 was generated from a synthetic peptide corresponding to amino acid 175-188 from human IKKε. This sequence is well conserved in rat and mouse IKKε.

*For more information, see UniProt Accession Q9UHD2
Mouse monoclonal purified with protein G chromatography is supplied in 100μl phosphate-buffered saline containing 0.05% BSA and 0.05% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects IKKε at 80 kDa* on SDS-PAGE immunoblots of human Jurkat cells. The antibody can be used for western blot and flow cytometry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY