Catalog # IM4761

IKKα Antibody

Mouse Monoclonal

Application Dilution
FC1:1000
IHC1:100
IP1:250
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 85 kDa

Isotype IgG1

DATASHEET  




$245

NF-κB (nuclear factor κB) is sequestered in the cytoplasm by IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKKα, IKKβ, and IKKγ. IKKα and IKKβ are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKKβ, not IKKα, is the target of proinflammatory stimuli. Targeted disruption of IKKβ gene in mice also results in extensive liver damage from apotopsis and death during embryo development.

 

References

Hu, Y. et al. (1999) Science 284:316.
Takada, K. et al. (1999) Science 284:313.
Delhase, M. et al. (1999) Science 284:309.

Western blot analysis of IKKα in human Daudi cells. The blot was probed with mouse monoclonal IKKα (IM4761) at 1:500.

Immunohistochemical analysis of IKKα in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control antibody (top left) and IKKα antibody (bottom left and right) at 1:100.



Clone 14A231 was generated from His-tagged full length IKKα. This sequence is well conserved in rat and mouse IKKα.

*For more information, see UniProt Accession O15111
Mouse monoclonal purified with protein G chromatography is supplied in 100μl phosphate-buffered saline containing 0.05% BSA and 0.05% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects IKKα at 85 kDa* on SDS-PAGE immunoblots of human Daudi and HEK293 cells. The antibody can be used for western blot, immunohistochemistry, immunoprecipitation, and flow cytometry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY