Catalog # IM3681

IKAP (Central region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 150 kDa

Isotype IgG1

DATASHEET  




$245

The NF-k B transcription factor coordinates a response to pathogens and pro-inflammatory cytokines, and is vital in the development of acute and chronic inflammatory diseases. NF-k B is activated by cytokine-activated IKB kinases (IKKs) found in large complexes. The IKK complexes consist of NIK, IKK-a, IKK-b , IkB-a, NF-k B/RelA and IKK-complex-associated protein (IKAP). First identified as a scaffolding protein of the IKK complex, IKAP, also known as elongation protein 1 (ELP1), has been linked to adhesion and migration in several cell types, including rat primary neurons. Depletion of IKAP by loss-of-function mutations in the IKBKAP gene has been shown to cause defective neuronal development and maintenance, leading to familial dysautonomia (FD). FD is a hereditary neuronal disease characterized by poor development and progressive degeneration of the sensory and autonomic nervous system. In addition, IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD.

 

References
Johansen, L. D. et al. (2008) Journal of Cell Science 121:854.
Naumanen, T. et al. (2008) Cel. Adh. Migr. 2(4):236.
Cohen, L. et al. (1998) Nature 395:292.

Western blot analysis of IKAP expression in human Jurkat cell lysate. The blot was probed with mouse monoclonal IKAP (Central region) at 1:500 (lane 1) or 1:2000 (lane 2).



Clone M368 was generated from a recombinant protein that included amino acid residues within the central region of human IKAP. This sequence has high homology with similar regions in rat and mouse IKAP.

*For more information, see UniProt Accession O95163
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 150 kDa* band corresponding to the molecular weight of IKAP on SDS-PAGE immunoblots of Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY