Catalog # IM2881

ILK (C-terminal region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 51 kDa

Isotype IgG1

DATASHEET  




$245

Integrin-linked kinase (ILK) is an important downstream kinase for integrin and growth factor pathways. ILK interacts with several proteins including integrins, PINCH, CH-ILKBP, and affixin, to coordinate signaling from integrin and growth factor receptors. ILK phosphorylates Akt at Ser-473, GSK-3 at Ser-9, and myosin light chain 2 (MLC2) at Ser-18/Thr-19 to regulate the activity of these key signaling elements. ILK is activated through PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP. Phosphorylation of ILK occurs at Thr-173, Ser-246, and Ser-343, and these modifications may be important for regulating ILK activity.

 

References
Persad, S. et al. (2001) J. Biol. Chem. 276, 27462-27469.
Morimoto, A.M. et al. (2000) Oncogene 19, 200-209.
Wu, C. and Dedhar, S. (2000) J. Biol. Chem. 155, 505-510.

Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-ILK (C-terminal region) antibody at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000 (lane 3).



Clone (M288) was generated from a sequence corresponding to amino acids in the C-terminal region of human integrin-linked kinase (ILK). This ILK sequence has high homology to rat and mouse ILK.

*For more information, see UniProt Accession Q13418
Mouse monoclonal, protein G purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 51 kDa* protein corresponding to the molecular mass of ILK on SDS-PAGE immunoblots of human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY